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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
03/03/2017 |
Data da última atualização: |
03/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARTINS, M. I. G.; SANT'ANA, A. E. G.; VASCONCELOS, F. M. T.; SILVA, W. L.; LIMA, L. M. de; CARVALHO, R.; MELO FILHO, P. A.; SANTOS, R. C. dos. |
Afiliação: |
MARIA ISABEL G. MARTINS, RENORBIO/UFRPE; ANTONIO EUZÉBIO G. SANT'ANA, UFAL; FELIPE MATHEUS T. VASCONCELOS, UFRPE; WBYRATAN L. SILVA, UFAL; LIZIANE MARIA DE LIMA, CNPA; REGINALDO CARVALHO, UFRPE; PÉRICLES A. MELO FILHO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
Título: |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
African Journal of Biotechnology, v. 15, n. 30, p. 1597-1607, July 2016. |
ISSN: |
1684-5315 |
Idioma: |
Inglês |
Conteúdo: |
Essential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut. MenosEssential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a sh... Mostrar Tudo |
Palavras-Chave: |
Aranha ácaro; Ocimum Basicilum L; Spider mites. |
Thesagro: |
Amendoim; Cromatografia; Tetranychus urticae. |
Thesaurus Nal: |
Chromatography; Eugenol; Peanuts. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156940/1/Bioactivity-of-basil.pdf
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Marc: |
LEADER 02601naa a2200325 a 4500 001 2065905 005 2017-03-03 008 2016 bl uuuu u00u1 u #d 022 $a1684-5315 100 1 $aMARTINS, M. I. G. 245 $aBioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut.$h[electronic resource] 260 $c2016 520 $aEssential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut. 650 $aChromatography 650 $aEugenol 650 $aPeanuts 650 $aAmendoim 650 $aCromatografia 650 $aTetranychus urticae 653 $aAranha ácaro 653 $aOcimum Basicilum L 653 $aSpider mites 700 1 $aSANT'ANA, A. E. G. 700 1 $aVASCONCELOS, F. M. T. 700 1 $aSILVA, W. L. 700 1 $aLIMA, L. M. de 700 1 $aCARVALHO, R. 700 1 $aMELO FILHO, P. A. 700 1 $aSANTOS, R. C. dos 773 $tAfrican Journal of Biotechnology$gv. 15, n. 30, p. 1597-1607, July 2016.
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Embrapa Algodão (CNPA) |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
22/09/2003 |
Data da última atualização: |
02/03/2015 |
Autoria: |
NEVES, E. J. M.; REISSMANN, C. B.; DÜNISCH, O. |
Afiliação: |
Neves, Pesquisador da Embrapa Florestas. |
Título: |
Distribuição de raízes e acúmulo de macronutrientes e AL+++ em espécies florestais plantadas em latossolo amarelo da Amazônia Brasileira. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Colombo: Embrapa Florestas, 2003. |
Páginas: |
20 p. |
Série: |
(Embrapa Florestas. Boletim de Pesquisa e Desenvolvimento, 13). |
ISSN: |
1676-9449 |
Idioma: |
Português |
Conteúdo: |
A formação e distribuição de raízes bem como o conteúdo de macronutrientes e AI foram estudadas em Ceiba pentandra e ViroIa surinamensis. As raízes foram separadas em raízes finas « 2 mm); casca e madeira das raízes grossas (> 2 mm) e casca e madeira da raiz pivotante. C. pentandra produziu 11,82 kg de biomassa seca de raízes, enquanto V. s'urinamensis 2,53 kg. Em C. pentandra estas distribuíram-se na seguinte ordem decrescente: madeira da raiz pivotante (6,66 kg); casca da raiz pivotante (2,98 kg); madeira da raiz grossa (1,49 kg); casca da raiz grossa (0,63 kg) e raiz fina (0,06 kq). enquanto em V. surinamensis a distribuição foi: madeira da raiz grossa (1,00 kg); madeira da raiz pivotante (0,82 kg); casca da raiz grossa (0,29 kg); raiz fina (0,25 kg) e casca da raiz pivotante (0,17 kg). Quanto aos elementos minerais, em C. pentandra os maiores acúmulos foram para: N nas raízes finas; K na casca e madeira das raízes grossas; Ca na casca e K na madeira da raiz pivotante. Em V. surinamensis estes foram promovidos por K nas raízes finas; AI na casca e K na madeira das raízes grossas; AI na casca e N na madeira da raiz pivotante. |
Palavras-Chave: |
Amazon region; Brasil; Ceiba Latossolo amarelo; Espécie florestal; Forest; Mineral nutrients; Nutrição mineral; Root; Sumaúma; Yellow latosol. |
Thesagro: |
Ceiba Pentandra; Espécie; Essência Florestal; Floresta; Latossolo Amarelo; Macroelemento; Planta; Raiz; Solo; Ucuuba; Virola Surinamensis. |
Thesaurus NAL: |
Amazonia; roots; Virola. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119093/1/digitalizar0021.pdf
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Marc: |
LEADER 02409nam a2200457 a 4500 001 1280999 005 2015-03-02 008 2003 bl uuuu u0uu1 u #d 022 $a1676-9449 100 1 $aNEVES, E. J. M. 245 $aDistribuição de raízes e acúmulo de macronutrientes e AL+++ em espécies florestais plantadas em latossolo amarelo da Amazônia Brasileira. 260 $aColombo: Embrapa Florestas$c2003 300 $a20 p. 490 $a(Embrapa Florestas. Boletim de Pesquisa e Desenvolvimento, 13). 520 $aA formação e distribuição de raízes bem como o conteúdo de macronutrientes e AI foram estudadas em Ceiba pentandra e ViroIa surinamensis. As raízes foram separadas em raízes finas « 2 mm); casca e madeira das raízes grossas (> 2 mm) e casca e madeira da raiz pivotante. C. pentandra produziu 11,82 kg de biomassa seca de raízes, enquanto V. s'urinamensis 2,53 kg. Em C. pentandra estas distribuíram-se na seguinte ordem decrescente: madeira da raiz pivotante (6,66 kg); casca da raiz pivotante (2,98 kg); madeira da raiz grossa (1,49 kg); casca da raiz grossa (0,63 kg) e raiz fina (0,06 kq). enquanto em V. surinamensis a distribuição foi: madeira da raiz grossa (1,00 kg); madeira da raiz pivotante (0,82 kg); casca da raiz grossa (0,29 kg); raiz fina (0,25 kg) e casca da raiz pivotante (0,17 kg). Quanto aos elementos minerais, em C. pentandra os maiores acúmulos foram para: N nas raízes finas; K na casca e madeira das raízes grossas; Ca na casca e K na madeira da raiz pivotante. Em V. surinamensis estes foram promovidos por K nas raízes finas; AI na casca e K na madeira das raízes grossas; AI na casca e N na madeira da raiz pivotante. 650 $aAmazonia 650 $aroots 650 $aVirola 650 $aCeiba Pentandra 650 $aEspécie 650 $aEssência Florestal 650 $aFloresta 650 $aLatossolo Amarelo 650 $aMacroelemento 650 $aPlanta 650 $aRaiz 650 $aSolo 650 $aUcuuba 650 $aVirola Surinamensis 653 $aAmazon region 653 $aBrasil 653 $aCeiba Latossolo amarelo 653 $aEspécie florestal 653 $aForest 653 $aMineral nutrients 653 $aNutrição mineral 653 $aRoot 653 $aSumaúma 653 $aYellow latosol 700 1 $aREISSMANN, C. B. 700 1 $aDÜNISCH, O.
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